We're interested in using WI-38 cells to establish an assay. We are interested in a human fibroblast cell line which is haploid and has been previously used in circadian experiments. The assay will require us to transfect or infect the cells and then to create clonal populations. Does anyone have experience with WI-38 cells and can speak to the ease/difficulty of working with WI-38 cells?
Dear jacqueline, Transfecting Wi 38 is done with great precaaution, i am sending you paper containing process of transfection kindly chk it in attachment.
all d best.
to follow-up, we are having success using the avalanche transfection reagent from EZ-biosystems http://ezbiosystems.com/view2.asp?d_id=208&name=wi-38%20cell%20ezfect%816%be4%20transfection%20reagent
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