Jacqueline Lane

4 Questions 6 Answers 0 Followers

Questions related from Jacqueline Lane

Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

02 November 2018 7,761 1 View

Is the 3T3-L1 cell line immortalized?

I'm wondering if the 3T3-L1 line is immortalized. I've seen conflicting reports where some papers call it immortalized and some do not. In addition, the ZenBio description sheet of the cell line...

19 May 2014 3,798 8 View

What is your preferred maxi prep kit for plasmid DNA grown in bacteria?

We currently use The HiSpeed Qiagen Maxi Prep kit. We are shopping around for other options. Does anyone have a preferred method/kit? How do yields compare? ease of method? cost? thanks!

13 February 2013 8,122 7 View

Does anyone have experience with WI-38 cells? Are they difficult to transfect? Can they readily form clonal populations?

We're interested in using WI-38 cells to establish an assay. We are interested in a human fibroblast cell line which is haploid and has been previously used in circadian experiments. The assay...

27 September 2012 7,976 2 View