Does anyone know a company that does Pyrosequencing to determine mitochondrial DNA mutation heteroplasmy?

We are looking to genotype our mice. Our mice have a mutation in their mitochondrial DNA and we want to know the level of heteroplasmy i.e. % of mutation in their mitochondrial DNA. So we cannot...

09 November 2020 4,246 3 View

Low yield with HCl in chalcone synthesis: Why?

Hello, I can't understand why a low yield is obtained with the use of HCl in aldol condensation in acid catalysis for the synthesis of hydroxychalcone.

28 July 2020 7,853 5 View

Why do I get the same model fit indices for bollen-stine bootstrap and ML in AMOS?

Hi Guys, I would highly appreciate some help/input regarding boolen-stine bootstraping in AMOS. Given that my data violated the multivariate normality assumption I have opted to run bollen-stine...

12 May 2020 2,391 3 View

Positive Zeta Potential NPs dispersed in water?

Hello guys I am looking for positive zeta potential colloidal particles dispersed in water which I can order online. pH around 6 to 7. The size its from 300 nm until 700 nm. Thanks a lot for...

28 March 2020 6,869 3 View

Does anyone have experience with antibodies from Invitrogen (Flow Cytometry)?

28 April 2019 2,718 2 View

Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

01 November 2018 7,642 1 View

Do mice have a dominant eye?

Do mice naturally have one eye that is more dominant than the other? The majority of people are right-eye dominant, and I was wondering if this could also be the case in mice.

08 May 2018 7,488 1 View

Tesr

Test

07 February 2017 6,051 3 View

Does anyone have spss syntax for d?

Does anyone have spss syntax for cohen's d in an independent T-test? Or can tell me how to do it elsewhere in SPSS?

19 January 2016 5,734 10 View

How can one make a dilution series before determining titer?

11 March 2015 7,875 3 View

WURSS-11 - how to apply in research practice?

Hello, in the framework of my master's thesis, I would like to assess the efficacy of probiotic on upper respiratory tract infection (URTI) incidence and symptom ratings in athletes under...

07 January 2021 2,284 1 View

Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Is there any highthrough put method for screening of microbes having Bsh activity ? #probiotics ?

can anybody help me with In-vivo screening strategy for microbes having Bsh activity? I want to explore them for cholesterol lowering activity?

11 October 2020 6,355 3 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

Apart from using probiotics, following biosecurity and good management practices, is there any cure/medicine for White feces disease in Vannamei now?

the gut related diseases like White feaces, white muscle, white gut are doing much damage to shrimp by retarding growth and increasing FCR and eventually heavy losses. is there any medicine or...

07 October 2020 5,213 3 View

Can anyone suggest which cell lines should be used to study probiotic adhesion study?

For probiotic adhesion study, many of the researchers used HT29 and Caco2 cell lines, among that which will be more preferred one

27 September 2020 968 4 View

Can anyone advise me in selecting a good pair of universal primer/primers for identification of lactic acid bacteria (probiotics)?

when I blast the existed primers specially 16s rDNAs , I cant get good results.

21 September 2020 1,584 4 View

Which animal model is better suited for hypercholesterolemia and probiotic studies?

We are planning an in vivo study on the relationship between hypercholesterolemia and probiotics. Unfortunately, we don't have the chance to work with hamsters, we have very few options for...

30 August 2020 8,794 6 View

How to make probiotic in different concentration?

I want to know how to make probiotic in different concentration. I am planning to probiotic experiment with different concentration. I also want to know how to make the freeze dry probiotic powder...

14 April 2020 1,197 2 View

How to get rid of from mold contamination from yeast culture on YEPD?

I am facing mold contamination on yeast culture on YEPD agar, i'm about screening yeast probiotics from wheat . please suggest me how to get rid of from this contamination?

09 October 2019 3,278 4 View