01 January 1970 2 2K Report

hi

Im gonna do a phage display selection with a membrane protein that is detergent stabilized. does anyone have a protocol for this or any experience in doing this?

are the phages effected? how well are detergent proteins immobilized with adsorption?

any help is greatly appriciated

More Gustav N Sundell's questions See All
How to remove unreacted florescent dye from labeled protein?

Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...

02 February 2021 644 2 View

Protocol for protein labeling using Rhodamine B Isothiocyanate?

Hi I want to label a protein with Rhodamine B Isothiocyanate which failed. I have the protein in PBS pH 7.2 the Rhodamine B Isothiocyanate dissolved in DMSO at 10 mM. added 15 times molar excess...

26 January 2021 7,468 3 View

Best practice for graphing linked data?

In an experiment with cardiomyocytes, each recorded cell was exposed to three different conditions. A) was a basal recording, B) was a low dose of a drug, and C) was a high dose of a drug. Every...

31 August 2020 3,246 1 View

How many nucleotides can you add in a PCR?

Hi so i have before added restriction sites and stop codons by flanking the primer with extra nucleotides. But now the question arises can I add more stuff this way? like a whole His-tag worth of...

24 November 2019 6,230 3 View

How do I bind Graphene to epoxy?

I am looking to use graphene sheets as strain gauges inside of a prepreg composite made of carbon fibers and epoxy resin (or BMI if easier). I haven't decided which kind of...

12 December 2018 6,073 3 View

Can you freeze diluted HRP-conjugated antibody?

Is it possible to reuse HRP conjugated antibody for ELISA after you have frozen(-20) the antibody that is diluted to experimental concentration?

18 May 2016 9,039 7 View

Similar questions and discussions
Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View

How can we study the adsorption efficiency of microgel at the oil-water interface?

I am studing the mircogel at oil-water interface. I want to know the adsorb amount of microgel at the oil water interface. How we can determine this amount?. I will highly appreciate your...

19 February 2021 2,787 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

NVT or NPT is preferable for gas adsorption simulation ?

Hi there, In order to perform molecule dynamic simulation on gas adsorption on substrate materials, NVT or NPT ensemble is preferable ? Thanks!

08 February 2021 173 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close