13 January 2014 0 2K Report

I would only like to know after how many days the knock down is most efficient in Huh7 hepatocytes.

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46 kDa band using a p65 (NFkB) antibody on rat tissue? JESS / Western Blot?

Hi guys, I am running WB on JESS (ProteinSimple) targeting p65 (NFkB) and we are, for some reason, detecting only a 46kDa band in the liver tissue. This is the cytosolic portion of the...

16 June 2024 4,759 0 View

What are the latest social justice issue for physician assisted suicide?

I am having trouble narrowing down the social justice framework of Physician Assisted suicide. thank you for your time. Nicole

09 June 2024 5,836 2 View

What method can I use to quantify trans-fatty acids in foods?

I'd like to measure trans fatty acids in industrial foods. What method of analysis would you recommend? I've heard of microwave-assisted extraction techniques for processing samples. My lab only...

30 April 2024 5,269 1 View

What would your recommendation for an EBP be for a student with ASD who is refusing to start assignments, although all are cognitively appropriate?

Student has HFA and is a 15 year old male

30 April 2024 3,887 2 View

How should microbiology specimens be processed in the laboratory to maintain integrity and accuracy?

Upon reading and understanding the article entitled “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar Reddy Kasarla and Laxmi Pathak, it was highlighted that specimen...

10 April 2024 4,653 3 View

How were sample quality, errors, values, and data evaluated during the transition to lithium heparin gel tubes in the clinical laboratory?

I'm eager for insights concerning the impact on sample quality and data integrity in the clinical laboratory.

11 March 2024 4,081 1 View

Can Rasch analysis indicate which items belong to a certain category?

Can Rasch analysis indicate which items belong to a certain category? I am trying to validate a survey about anxiety. The Likert scale is from 1 to 6 for each of the 17 items. My goal is to...

22 February 2024 6,376 3 View

What would be the appropriate research design if the subject of your study is an inanimate object e.g microbial fuel cell?

I keep getting confused that if it's true experimental design, who would answer the pre and post test?

06 February 2024 8,577 1 View

Can I call inanimate objects as participants?

I am conducting an on-going research study and I am evaluating the performance of consortium of bacteria as a biocatalyst in a microbial fuel cell. There's some misunderstanding between me and the...

05 February 2024 8,252 1 View

What method is better than Indirect Ion Selective Electrode when measuring parameters like Sodium, Potassium, and Chloride?

I am looking for other alternative methods to measure the electrolytes aforementioned since other studies claim that Indirect ISE can induce error in its results due to its dilution step.

24 January 2024 7,519 4 View

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How to avoid errors while doing qpcr?

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