My transduced cell can resist Puromycin and 99% express GFP, BUT can not show me the difference in the knockdown. what could be the possible reason?

More Mansur Dabai Salisu's questions See All

What are the possible reason(s) that make AsPC-1, 293T and MCF-7 cell lines failed to be transfect/transduced?. Please help me with possible hints?

what are the possible reason(s) that make AsPC-1, 293T and MCF-7 cell lines failed to be transfect/transduced?. Please help me with possible hints?

10 March 2024 8,811 2 View

Is it possible perovskite with Flourine will have lower band gap compared to Cl ?

I have calculated band structure of a halide perovskite consisting Flourine but its gap is lower than the perovskite with Cl how is this possible?

28 February 2024 1,527 1 View

If the melting temparature of a half heusler is below 1200 degree celcius, can we use it application? i saw various compounds having temp above 1500 ?

nothing

08 November 2023 5,318 0 View

I have another question. I have investigated phonon dispersion & there is a negative value -.0005. Does it still consider as dynamically unstable??

Phonon dispersion problem related to half heusler compound

21 October 2023 4,928 3 View

How can i dissolve PALMITIC ACID?

Please i have been trying to dissolve Palmitic acid in BSA-NaOH, it keeps solidifying after heating. I wanted to used DMSO but its' lethal to cells. And also the DMSO recommended concentration to...

25 September 2023 989 1 View

If i want to calculate heat capacity for half Heusler compound is the heat capacity equation will be same i used in halaideperovskite compound in DFT?

18 September 2023 6,997 0 View

Is it possible for a semiconductore to increase band gap while applying pressure then after certain pressure ,then band gap will decrease again?

Half heusler alloy electronic property under pressure

12 August 2023 5,002 3 View

Please can someone help me with the link to download to NTSYSpc software?

We're performing genetic diversity studies using SSR and RAPD markers and we're done with the experiments, but we're finding it difficult to get this software for the data analysis.

06 May 2023 6,728 3 View

Can I get guiding documents for broth microdilution and agar dilution methods?

I would greatly appreciate someone assisting me with guiding documents for broth microdilution and agar dilution. Thank you.

24 November 2022 361 1 View

Why do we determine maximum absorption of a complex compound?

For a synthesized complex compound, why do determine its maximum absorption using spectrophotometer?

17 February 2021 7,543 3 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,628 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,160 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,986 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,289 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 588 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,891 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,005 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 365 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View