Can I perform a Benjamini Hochberg FDR correction over p values from different tests?

I'm performing statistical analysis on a time series data, comparing the average amplitude (y axis) between three groups on every 50 ms (x axis). The time series is 1200 ms long (so in total 24...

21 October 2020 3,269 3 View

Please, I need clarification on the quality status of a journal listed in WoS Zoological Record. Is this index comparable to SSI, ESCI, etc?

So many journals claim they are indexed in WoS (ISI in old parlance; now called Clarivate Analytics). However, most of the journal end up listed in the Zoological Record (ZR). Reading about that...

03 March 2020 1,733 3 View

Hello ! Is it possible to use genomic DNA(gDNA) to check gene expression instead of using cDNA ?

Actually I m doing gene expression study and thought that we can save our time to directly use gDNA to check gene expression instead of following long protocols of RNA isolation and cDNA making

01 April 2018 8,747 5 View

Which simulator is best suited for IoT security?

15 March 2018 1,644 3 View

Can you suggest any validated measure of trait emotional intelligence (trait EI) that has the fewest possible number of items?

The measure should preferably be domain-specific to teacher-leader trait EI.

03 April 2017 2,398 5 View

Why, when using FHI-aims, did I find series of frequencies (about 300 of them) with more than half of them negative?

11 June 2015 6,526 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View