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I need a guideline for establishing very large broiler farms and the facilities required, emphasizing the size of the houses and the appropriate spacing of the houses from each other.
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I am performing transformation for the L4440 (2790 bp) plasmid into DH5 Alpha, but no colonies are forming. I performed the same procedure using PUC19 and observed colony formation. My competent...
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A study on customer perception towards choosing a life insurance company with special reference to Bharti AXA Life Insurance
15 August 2025 8,862 0 View
Biochemistry: How do post-translational modifications affect protein folding and function in neurodegenerative diseases?
09 August 2025 8,522 3 View
Utility functions are the core of many Neoclassical (New Classical, New Keynesian) models. Representative consumers (agents or families) choose a bundle of consumption goods and services which...
05 August 2025 2,148 10 View
Why is membrane selectivity often prioritized over permeability in practical gas separation applications? Recommendations for relevant research articles are appreciated.
01 August 2025 7,393 2 View
I have a pHelper plasmid that is 13kb. I have successfully gotten it to grow using DH5alpha cells in the past. There usually weren't many colonies and I would either need a large volume of culture...
10 July 2025 3,865 2 View
I’m performing a CRISPR knock-in to introduce a FLAG tag at the C-terminus of a gene, just before the stop codon. Due to guide RNA design constraints, my guide cuts approximately 80 bp upstream of...
15 June 2025 460 0 View
I'm quite new to doing qPCR and after optimising my target of interest and housekeeper primers, I've ended up with them having differing optimal annealing temperatures. How do I run my actual...
09 June 2025 1,467 2 View
WHO Pandemic treaty is based mainly on Vaccine and Pharmaceutical equality. It also suggests Pathogen access and Virus sharing for research in vaccine, therapeutics and diagnostics. However, the...
01 June 2025 147 2 View
Dear Colleagues, We kindly invite you to take part in a brief survey on digital reconstructions of ancient polychromy. The survey aims to gather expert input from archaeology, art history,...
23 May 2025 2,611 2 View
Can anyone share, primer sequence for PCR to confirm pFAST Bac Dual plasmid inserted in DH5alpha cells? probably for polyhedrin promoter
22 April 2025 8,333 2 View
How do I find my ResearchGate ID number?
21 April 2025 2,409 0 View
I am currently thinking of initiating a research project to develop a patented, ready-to-manufacture 200 MW turbine generator powered by natural gas, focusing on innovative turbine stage...
19 April 2025 6,459 1 View
When a user asks a question and his question is ambiguous to the system, the system should ask the user some clarification questions to accurately determine the user's intention? Can this be done...
19 April 2025 6,549 1 View
Good morning, My name is Fayez. I am in the first year of the PhD program in Mechanical Engineering at the University of Sheffield. I am working on CO2 capture from a natural gas power plant, and...
18 April 2025 7,415 0 View
The STEM Modeling Competency scale or the Modeling Ability Checklist would also be greatly appreciated if anyone has access to them!
13 April 2025 2,822 1 View
Need suggestions for reputable journals in the area of HRM and business management. Even , list of international peer-reviewed which is open access and listed in Europe or America.
10 April 2025 4,194 0 View
I tried to culture chlorella in three different pH (acidic, neutral, basic) using a medium. Unusual foaming was observed in 3 days. The remaining medium was stored in the same condition and the...
02 April 2025 3,959 1 View
Hi, I am measuring single cell evoked EPSPs in neurons from acute slices. However, sometimes the network activity can disturb my measurements. I am looking to presynaptically suppress this...
25 March 2025 4,531 2 View
I performed the opt + freq in xtb using the following keyword: ''--ohess --alpb water" the optimization ran fine with NO message like this one:...
23 March 2025 5,382 1 View
It is enough to know both languages and the linguistic ability which will enable you to practice translation as a profession. On the other hand, teaching translation is different, as (Gabris,...
11 March 2025 9,116 2 View
According to Mayne’s paper "Robust Model Predictive Control of Constrained Linear Systems with Bounded Disturbances", when considering a nonzero initial error, i.e., x∈x0⊕Z, the computation of ZZ...
06 March 2025 7,960 0 View
discuss in detail, data collection methods and tools used in the context of tourism product development(explain how they work, types and examples)?
01 March 2025 5,101 1 View