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Questions related from Nicole Paland
I have designed primers using the mRNA sequences provided by ncbi with Primer3 and primer blast from ncbi for my target genes. I have checked those primers with in silico PCR with USCS and they...
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Dear All, I would like to check the cell viability of THP-1 derived macrophages after treatment with different stimulants. I am looking for a specific gene that is preferably upregulated in these...
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I want to add glucosylceramide to cells. I redissolved the lipid obtained from Avanti Polar Lipids in absolute ethanol to obtain a 100 mM solution. From this I took 4 ul in PBS and sonicated 30...
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We would like to add glucosylceramide and glucosylsphingosine to neuronal cells, (cell line later primary cells). I am interested in detailed protocols, specifically concerning resuspension,...
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I want to perform co-localization studies. My secondary antibody anti-rabbit Cy2 has a very high background. Actually, there is no difference between only secondary antibody negative control and...
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I would only like to know after how many days the knock down is most efficient in Huh7 hepatocytes.
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How do people usually check if mTORC1 is activated? By checking if S6K1 is phosphorylated? How does mTORC1 get activated? Also by phosphorylation or is it transcriptionally regulated?
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We have major problems detecting sr-b1 in Huh7 cells (liver cells) using the antibody from NB400-104 from Novus Biologicals. Does anybody have experience with this antibody concerning lysis of the...
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