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Questions related from Nicole Paland
I have designed primers using the mRNA sequences provided by ncbi with Primer3 and primer blast from ncbi for my target genes. I have checked those primers with in silico PCR with USCS and they...
20 April 2021 9,822 3 View
Dear All, I would like to check the cell viability of THP-1 derived macrophages after treatment with different stimulants. I am looking for a specific gene that is preferably upregulated in these...
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I want to add glucosylceramide to cells. I redissolved the lipid obtained from Avanti Polar Lipids in absolute ethanol to obtain a 100 mM solution. From this I took 4 ul in PBS and sonicated 30...
28 January 2015 9,674 5 View
We would like to add glucosylceramide and glucosylsphingosine to neuronal cells, (cell line later primary cells). I am interested in detailed protocols, specifically concerning resuspension,...
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I want to perform co-localization studies. My secondary antibody anti-rabbit Cy2 has a very high background. Actually, there is no difference between only secondary antibody negative control and...
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I would only like to know after how many days the knock down is most efficient in Huh7 hepatocytes.
13 January 2014 1,860 0 View
How do people usually check if mTORC1 is activated? By checking if S6K1 is phosphorylated? How does mTORC1 get activated? Also by phosphorylation or is it transcriptionally regulated?
31 December 2012 7,890 2 View
We have major problems detecting sr-b1 in Huh7 cells (liver cells) using the antibody from NB400-104 from Novus Biologicals. Does anybody have experience with this antibody concerning lysis of the...
19 April 2012 858 6 View
