How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

How to set spring constant for umbrella sampling in GROMACS?

Hello everyone, I'm new to gromacs and also new to umbrella sampling, so I'm following Justin's tutorial http://www.mdtutorials.com/gmx/umbrella/index.html ; On the umbrella sampling stage, I'm...

20 February 2020 1,781 3 View

VMD selection (by position) isnt considering whole molecules?

Hi all, I've been trying to select a small water slab from my sistem (.pdb file containing a POPC bilayer in water) and moving it, but when I do it I notice some of the water molecules of the...

05 December 2019 4,159 4 View

State with plural ethical background?

Let A be a person with a consequentialist ethics and B with a deontological ethics. Is it possible that A and B agree on the structure of the State? If so, how would they reach an agreement?

02 December 2019 5,019 6 View

Minimum distance between ITRs in AAV for efficient transgene expression?

I'm just trying to express a very small transgene by transfecting cells with a plasmid coding for the recombinant genome of AAV2. I have different constructs with different promoters, and there is...

17 July 2018 5,903 6 View

Hi, could anyone give me some advice to clean the glassware from the excess of polymer urea-formaldehyde ? Thanks

The cleaning of the glassware after the synthesis of PUF particles is very difficult and almost impossible to remove completely the polymer attached to the walls, neither with acids, neither NaOH...

14 May 2017 5,577 3 View

No title

Hi, Some weeks ago I sent some paraffin blocks and sections (mouse tissues from a very important experiment) to another lab and just today I was informed that the person who received the package...

03 January 2017 6,247 6 View

Comparison of goodness-of-fit measures between MPLUS and LISREL. Why does the value of CFI, using a CFA, vary from LISREL to MPLUS?

16 November 2015 2,137 5 View

Should we use a 5 or 7 point Likert scale? What's better and why?

As researchers do not agree with the appropriate number of point Likert scale (5, 7...?), I would like to know the main contributions or papers to support both options.

19 January 2015 9,253 42 View

Can anyone share a key for identification of species of Heliothinae moths using genitalia?

Helicoverpa zea , armigera, gelotopoeon, bractae..

15 January 2015 5,888 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View