I need to do a calibration line of 4-nonylphenol in methanol with a HPLC-MS. The main problem is the lowest detection limit that I obtained was very high (around 0,1 ppm), so is impossible to use this technique for detect this contaminant in my samples.
The method used was:
- Mobile phase: water (A) + methanol (B)
- Flow rate: 0,4 mL/min
- Gradient: started with a 60% of A and 40% of B and was increased to 100% of B in 8 minutes. These conditions was maintained during 2 minutes.
Is possible to detect lower concentrations with another method? Is necessary make a previous preparation of the samples (ex: pass through a cartridge the sample, adjust the pH, add another component...) in order to improve de detection limit of the equipment?
Dear Noelia,
thanks for posting this very interesting technical question and sorry to see that it has not yet received any expert answers. I would not call myself an expert in this field. Our research interests are mainly in organometallic chemistry. Thus all I can do right now is suggest to you some potentially useful articles which might help you in your analysis. Please have a look at the following papers:
HPLC-FLD determination of 4-nonylphenol and 4-tert-octylphenol in surface water samples
Article HPLC-FLD determination of 4-nonylphenol and 4-tert-octylphen...
and
Extraction of 4-octylphenol and 4-nonylphenol in river water using solid phase extraction and high-performance liquid chromatography
Article Extraction of 4-octylphenol and 4-nonylphenol in river water...
Both articles are freely available as public full texts on RG and can be downloaded as pdf files. It's also worth a try to search the "Publications" section of RG for other helpful literature references.
Good luck with your work!
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