How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

How to measure a Neural Network complexity?

Does anyone know how to measure the complexity of an artificial neural network? Could you provide me references? Thanks a lot!

25 January 2021 2,710 10 View

State with plural ethical background?

Let A be a person with a consequentialist ethics and B with a deontological ethics. Is it possible that A and B agree on the structure of the State? If so, how would they reach an agreement?

02 December 2019 5,019 6 View

What is the best buffer and method for antigen retrieval of lymphoid cell surface markers such as CD3, CD4 and CD8 by immunohistochemistry approaches?

We are trying to achieve better results in our immunohistochemistry assays (paraffin-embedded tissue sections) for antigen retrieval of surface markers such as CD3. Our current protocol of sodium...

08 June 2017 7,048 6 View

Why light, at "that" speed, does not become matter?

Remember E=mc2

09 April 2017 1,326 11 View

How to recover the original color of a beetle that was killed in alcohol?

Dear colleagues, I have a Cerambycidae beetle that was killed in alcohol and I think that their original color was clearer... Can I dip it in benzene? Thanks, Italo.

04 December 2016 7,059 13 View

Which DMT system assumption is broken when using impulse responses with ringing for a time-domain transmission-based simulation?

18 November 2014 8,429 3 View

Are there evolution equations admitting peakon solutions?

It is well known that certain integrable equations such as the Camassa-Holm, Novikov and Qiao admit the so-called peakon solutions. However, all of them are not evolution equations. Then I would...

14 November 2014 1,197 2 View

What is the general formula for adjoint differential operator and the associated vector field?

I am working with high order PDEs and I need to know the expressions of the adjoint operator once the linear operator is given.

05 May 2014 2,415 3 View

Does anyone have experience with blue transfer buffer?

I've been doing western blot the last two years and I have never had a problem like this. When the transference is finished, the transfer buffer has turned blue. It seems that the sample buffer...

27 January 2014 3,493 1 View

Why are readings on an Eppendorf BioFlo 120 bacterial bioreactor lost after a few hours?

Hello all, Our lab has a bacterial bioreactor from Eppendorf (BioFlo 120) that is normally set to read/record trend data (pH, temperature, etc.) over a set number of hours (overnight). We...

26 January 2021 8,192 1 View

How to de-clump HEK293 suspension cells?

I have a problem with clumping of HEK293 suspension cells, and this clumping problem decreases the cell viability. I will transfect the cells if I were to reach a favorable cell count. What can I...

17 January 2021 354 4 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

Best Way to Preserve Nitrifying Bacteria in Liquid Culture?

I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...

18 December 2020 5,411 4 View

How Can I convert OD550 (Optical Density at 550 nm) to NTU (Nephelometric Turbidity Unit)?

OD and NTU are two different units, that can be measured through a spectrophotometer and Bioreactor (integrated with turbidity sensor) respectively. Because of the difference in units, the results...

03 October 2020 5,662 2 View

How to draw a operating window to demonstrate the bioreactor performance?

I would like to make a operating window for a bioreactor process where I want to incorporate data such as impeller speed, gas flow rate, hydrodynamic shear, mass transfer efficiency, power...

01 September 2020 2,501 3 View

Hi, i want to scale up my recombinant protein production from flask to 5L fermentor can i use my optimized condition in the flask for fermetor?

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12 July 2020 5,340 3 View

Can anybody suggest how to calculate the volume of a fed-batch bioreactor?

I've done mass balance around the reactor but I'm stuck at the volume bit because volume is not constant.

28 March 2020 8,702 3 View

Can trial reactors be setup without replicate (duplicate) in semi-continuous long term AD experiments?

I am planning a new set of AD experiments (agricultural waste as a substrate), and if I run a duplicate of my trials, I will have a total of 6 reactors (this seems a lot of work since I have to...

18 February 2020 6,904 5 View

Can trial reactors be setup without replicate (duplicate) in semi-continuous long term AD experiments?

I am planning a new set of AD experiments (agricultural waste as a substrate), and if I run a duplicate of my trials, I will have a total of 6 reactors (this seems a lot of work since I have to...

01 February 2020 10,072 4 View