I have cryopreserved the undifferentiated condition of SH-SY5Y cell line in the early passage in a cyomedia formulated with 90% FBS and 10% DMSO (sterile-filtered). Some months later, I have thawed the cryopreserved undifferentiated cell line, but what I found in the laboratory is the undifferentiated cell line are fully recovered in a differentiated condition, the cells were grown in a regular maintaining growth media (DMEM/F12 supplemented with 10% FBS and without any of the differentiating agent). Is it possible to get the cells back into its undifferentiated condition? Can anyone give me a suggestion related to this? thank you.
When SH-SY5Y is cultured at a low density, it spontaneously produces neurites. Although it begins to show some differentiation markers, it is not true differentiation. By providing optimal culture conditions, you can revert them from this false differentiation to an undifferentiated state. For more details, read: https://www.nature.com/articles/s41598-024-63005-y.
Again, ATCC recommends freezing SH-SY5Y cells in 5% DMSO. Always follow the best conditions possible.
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