Does anyone have any scoring systems for liver and kidney histopathological evaluation?
Hi Riva
I have experience in toxicity scoring only. My score system for toxicity based on the hepatocyte in Kiernan zone (liver) or epithelial cell in proximal duct (kidney), as below
parametric:
1. nucleus : normal (0), anisocytosis (1), karyopyknosis (2), necrosis (3)
2. cytoplasm: normal (0), hydropic degeneration (1), lipid degeneration (2)
By that scoring i usually use R software for clustering and add heatmap for maping the lesion. by grouping system we will get the group with severe, moderate, mild lesion and normal
good luck in your research
please find file attachment, these file is example of clustering method in liver lesion by histopathology but in fibrosis modeling research
For histopathological evaluation in our study, the largest lobe of the liver was fixed in 10% neutral buffer formalin, embedded in paraffin and 5micrometer thick section was used for H&E staining. The scanned images were analyzed and gross morphological alterations could be seen. For an unbiased scoring, the identity of slides were blinded and evaluated (by pathologist) for histopathological lesions (necrosis, fat infiltration, fibrosis and mononuclear cell infiltration) and given a score that ranged between 0 (60%). The lesions observed fell under following categories viz none (score=0), minimal (score=1), mild (score=2), moderate (score=3) and marked (score=4). Further, the distribution of these lesions were also classified as focal, multifocal and diffuse.
Hope this may provide some help!
Thank you so much Rashmi Pathak and Eric Kouba.
May I know from what textbook or article was the basis of your scoring method? And is it possible for you to attach if there is a file of such scoring.
Thank you so much.
I happen to find another scoring method for liver which is based from Ishak et al. (1995) and the R.E.N.A.L. scoring method for kidney. I just don't know if I will be using the correct scoring for the mentioned organs. What do you think? Thank you.
Mawar Subangkit can u plz explain what is heat map..cn this method used for scoring lesions for brain gills and intestine too
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