Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the beginning. I've been trying to do a viability assay but I just can't get consistent results mainly because the they don't behave the same in every well. In some they form a layer and are good to use in others they are either ir clusters or dead. This happened in the the 96 and 24 well plates. In the flasks and 6 well, that my colleague is doing they star to adhere within one hour. I've asked this colleague do same plates for me as I thought it was a problem with the way I was handling them but to no avail. I've also increases the number of cells per well and nada
Does anyone have any advice?