What about the not-so-talked-about Raman peaks of graphene/graphite?

I'm trying to analyse my Raman spectra of reaggregated graphene samples for any signs of functionalisation, and I know this can normally be deduced from the ratio of the D to the G peaks and the...

31 August 2016 4,047 4 View

How much dexamethasone should I use on THP-1 cells?

I want to create a dose-response curve to show the effect of dexamethasone on inflammation in THP-1 cells. However, I can't seem to find appropriate concentrations of dexamethasone to try in any...

23 September 2015 4,837 2 View

What are some efficient methods for digestion of Selenomethionine with endpoint analysis being detection of pure Selenium?

Many methods available however most involve nitric acid.

17 June 2014 8,783 2 View

How to add a colour legend onto heatmap in R?

I have been using the R statistics package to display a heatmap of Illumina sequencing data (imported as a csv file of the sample names, species names, and the % abundance). I have managed to get...

13 October 2013 9,053 15 View

Why is science Ignoring the inner force or spirit

I'm trying to write a critical analysis paper that addresses a problem in motivational science. Since science largely ignores the spiritual component, I think this would be an acceptable topic...

01 January 1970 312 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View