Does anyone have any recommendations for the successful depletion of Caco-2 Glutatione levels by BSO in a 96 well-plate (adherent culture)?

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Marwa Ezz ElDeen Sayour

Dear Ciara, BSO will be supplied to cultures 8 hours after plating. Serum-starved cells will be treated with BSO for 24 hr. Thereafter, BSO will be removed and replaced with fresh medium (Schnelldorfer et al., 2000).

Ciara Mckinley

Dear Marwa,

Thank you very much for your answer. I was waiting the traditional 24hr plating time. I will try with the shorter time of 8hrs. Thank you so much again!

My pleasure