Could you recommend chemical inducers of senescence in human mesenchymal stem cells?

More Ciara Dean's questions See All

What are the latest approaches to integrating religious education in today’s curriculum reforms?

The ERATS July 2024 Special Issue is now inviting submissions under the theme ‘Religious Education in a Curriculum Restructuring Era.’ This issue aims to explore the dynamic interplay between...

09 April 2024 8,201 2 View

How effective is community-driven development?

existing community-driven development projects in the Philippines

22 March 2024 8,346 0 View

How to slow down 1165 based lift-off for thermally deposited metals on semiconductors ?

quick summary: I am looking for a way to slow down lift-off (1165 or acetone) for a given material system (changing materials to solve the problem is not possible) This is a relatively general...

08 January 2024 8,955 2 View

Qubit vs Quantus, which is better and why?

Current application is library quantitation for NGS.

05 April 2023 317 1 View

[LAMMPS] How/Where can I get a potential for Germanium-Arsenic and Silicon-Arsenic interaction?

I am planning to simulate the diffusion of As atoms (as dopants) within a growing Ge layer. The system can be pictured as a flux of As and Ge atoms being deposited on a Silicon substrate. The...

29 March 2023 1,458 1 View

What is considered the most affordable sequencing method for identifying bacteria in urban rivers?

My undergraduate thesis partner and I will be identifying and characterizing the microbial diversity in 3 sample sites from an urban river. We were thinking of using Sanger sequencing since we...

13 March 2023 2,629 3 View

Can qPCR products be sequenced in Ampliseq workflow?

We frequently have mixed results for a qPCR assay. We'd like to determine if this is non-specific amplification, or target amplification when the target is low-frequency. DNA tape-station shows...

02 February 2023 6,777 5 View

RQ-PCR, what are these mysterious false positives?

Our lab has an assay to detect fusion transcripts that very frequently results in "false positives." Often our duplicates show mixed results (one positive and one negative), always at a high C(t),...

09 January 2023 2,609 3 View

How is movement on a thrust sheet accommodated form immediately adjacent to, but out side of the sheet?

Every reference paper I have viewed on thrust faulting shows geologic cross section parallel to the direction of movement of the thrust sheet. My question is what do perpendicular cross sections...

27 July 2022 616 0 View

To what extent have customs (customary law) and general principles been influential in the development of international criminal law?

Some scholars argue that the notion of customary law as a source of international law is questionable on the premise that customary law is broad and vague, hence its obscurity is discrepant with...

15 October 2021 2,266 10 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 640 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,893 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View