I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the same?
Protein transfection is generally less efficient (given the size of proteins) but can accomplished through several means;
- Electroporation, although with larger proteins this can be brutal and negatively affect cell viability
- Lipofection, where proteins are encapsulated by liposomes, which then fuse with the cell membrane to release the proteins
- Viral Transfection, but that is usually done with nucleic acid sequences, not necessarily complete proteins.
I think you are referring to RNAi technology as it is implicated in protein targeting - transfecting siRNA to silence a given protein is a well-established practice. Shorter sequences of RNA can easily be introduced in a non-cytotoxic manner using chemical transfection reagents (see https://altogen.com/product/lipid-in-vivo-transfection-reagent/), although you will have to make sure that the reagent you select is compatible with the cells you are using. Some cells (like neurons) are notoriously difficult to transfect, and may require viral-mediated mechanisms. Others, like liver cells, can be efficiently targeted using appropriate cell markers. There are many commercially available reagents for cell-line specific siRNA transfection, so choose the one that corresponds to the cells you are using.
Hii Kanika Jain you can transfect your protein easily by conjugation with polymeric nanoparticles or micellar system we are working on preparation of such types of carrier molecules.
Protein transfection is generally less efficient (given the size of proteins) but can accomplished through several means;
- Electroporation, although with larger proteins this can be brutal and negatively affect cell viability
- Lipofection, where proteins are encapsulated by liposomes, which then fuse with the cell membrane to release the proteins
- Viral Transfection, but that is usually done with nucleic acid sequences, not necessarily complete proteins.
I think you are referring to RNAi technology as it is implicated in protein targeting - transfecting siRNA to silence a given protein is a well-established practice. Shorter sequences of RNA can easily be introduced in a non-cytotoxic manner using chemical transfection reagents (see https://altogen.com/product/lipid-in-vivo-transfection-reagent/), although you will have to make sure that the reagent you select is compatible with the cells you are using. Some cells (like neurons) are notoriously difficult to transfect, and may require viral-mediated mechanisms. Others, like liver cells, can be efficiently targeted using appropriate cell markers. There are many commercially available reagents for cell-line specific siRNA transfection, so choose the one that corresponds to the cells you are using.
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