In rat platelet samples
Did you try the Emfret JON/A antibody? Although its a rat antibody, you might try the directly conjugated antibody.
I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the...
01 February 2017 8,235 4 View
Can someone please suggest a protocol for the preparation of rat tissue lysates to perform immunoprecipitation? Is the sample preparation same as for western blotting?
10 November 2013 3,090 2 View
I came across the use of scoring in immunohistochemistry. Can someone please help me understand how I perform this scoring in tissue section for my DAB stained sections?
10 November 2013 2,369 4 View
Is it possible to perform immunoprecipitation assays using antibodies which are specified for WB or ELISA?
10 November 2013 7,061 3 View
Using gelatin zymography for cardiac tissue homogenates.
06 July 2013 8,068 2 View
i wish to use an ex vivo rat heart model to identify the effect of er stress inducers following treatment of the animals with an antioxidant. I need help in the buffer and perfusion composition...
06 July 2013 4,068 0 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...
24 February 2021 974 1 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...
21 February 2021 8,548 7 View