Kanika Jain

7 Questions 6 Answers 0 Followers

Questions related from Kanika Jain

Does anyone have any experience with Protein transfection?

I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the...

02 February 2017 8,275 4 View

How to prepare samples for co-immunoprecipitation in tissues?

Can someone please suggest a protocol for the preparation of rat tissue lysates to perform immunoprecipitation? Is the sample preparation same as for western blotting?

11 November 2013 3,128 2 View

Suitability of primary antibodies for immunoprecipitation?

Is it possible to perform immunoprecipitation assays using antibodies which are specified for WB or ELISA?

11 November 2013 7,103 3 View

Scoring in Immunohistochemistry?

I came across the use of scoring in immunohistochemistry. Can someone please help me understand how I perform this scoring in tissue section for my DAB stained sections?

11 November 2013 2,415 4 View

Does anyone have a standardised protocol for ex vivo rat heart model?

i wish to use an ex vivo rat heart model to identify the effect of er stress inducers following treatment of the animals with an antioxidant. I need help in the buffer and perfusion composition...

07 July 2013 4,142 0 View

How can I detect activated rat alpha(IIb)beta(3) by flow cytometry?

In rat platelet samples

07 July 2013 318 1 View

Can someone share a standardised protocol for calpain (gelatin) zymography?

Using gelatin zymography for cardiac tissue homogenates.

07 July 2013 8,108 2 View