Can someone share a standardised protocol for calpain (gelatin) zymography?

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Does anyone have any experience with Protein transfection?

I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the...

01 February 2017 8,325 4 View

How to prepare samples for co-immunoprecipitation in tissues?

Can someone please suggest a protocol for the preparation of rat tissue lysates to perform immunoprecipitation? Is the sample preparation same as for western blotting?

10 November 2013 3,164 2 View

Suitability of primary antibodies for immunoprecipitation?

Is it possible to perform immunoprecipitation assays using antibodies which are specified for WB or ELISA?

10 November 2013 7,142 3 View

Scoring in Immunohistochemistry?

I came across the use of scoring in immunohistochemistry. Can someone please help me understand how I perform this scoring in tissue section for my DAB stained sections?

10 November 2013 2,453 4 View

How can I detect activated rat alpha(IIb)beta(3) by flow cytometry?

In rat platelet samples

06 July 2013 358 1 View

Does anyone have a standardised protocol for ex vivo rat heart model?

i wish to use an ex vivo rat heart model to identify the effect of er stress inducers following treatment of the animals with an antioxidant. I need help in the buffer and perfusion composition...

06 July 2013 4,199 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Jofre Font-Mateu

I've using this one and as you can see it worked quite well

cheers

https://www.researchgate.net/publication/7661127_Improved_surgical_mesh_integration_into_the_rat_abdominal_wall_with_arginine_administration?ev=prf_pub

Article Improved surgical mesh integration into the rat abdominal wa...

Debora Fontanini

Hi Kanika, I've used for years the method described/cited in this paper. It's been working very well for me.