Can someone share a standardised protocol for calpain (gelatin) zymography?

More Kanika Jain's questions See All

Does anyone have any experience with Protein transfection?

I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the...

01 February 2017 8,235 4 View

How to prepare samples for co-immunoprecipitation in tissues?

Can someone please suggest a protocol for the preparation of rat tissue lysates to perform immunoprecipitation? Is the sample preparation same as for western blotting?

10 November 2013 3,090 2 View

Scoring in Immunohistochemistry?

I came across the use of scoring in immunohistochemistry. Can someone please help me understand how I perform this scoring in tissue section for my DAB stained sections?

10 November 2013 2,369 4 View

Suitability of primary antibodies for immunoprecipitation?

Is it possible to perform immunoprecipitation assays using antibodies which are specified for WB or ELISA?

10 November 2013 7,061 3 View

How can I detect activated rat alpha(IIb)beta(3) by flow cytometry?

In rat platelet samples

06 July 2013 278 1 View

Does anyone have a standardised protocol for ex vivo rat heart model?

i wish to use an ex vivo rat heart model to identify the effect of er stress inducers following treatment of the animals with an antioxidant. I need help in the buffer and perfusion composition...

06 July 2013 4,068 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Jofre Font-Mateu

I've using this one and as you can see it worked quite well

cheers

https://www.researchgate.net/publication/7661127_Improved_surgical_mesh_integration_into_the_rat_abdominal_wall_with_arginine_administration?ev=prf_pub

Article Improved surgical mesh integration into the rat abdominal wa...

Debora Fontanini

Hi Kanika, I've used for years the method described/cited in this paper. It's been working very well for me.