Suitability of primary antibodies for immunoprecipitation?

More Kanika Jain's questions See All

Does anyone have any experience with Protein transfection?

I want to try doing a protein transfection experiment to target my protein of interest. there are are reagents like ProteoJuice which can be used. Does anyone have any experience or insight on the...

01 February 2017 8,235 4 View

How to prepare samples for co-immunoprecipitation in tissues?

Can someone please suggest a protocol for the preparation of rat tissue lysates to perform immunoprecipitation? Is the sample preparation same as for western blotting?

10 November 2013 3,090 2 View

Scoring in Immunohistochemistry?

I came across the use of scoring in immunohistochemistry. Can someone please help me understand how I perform this scoring in tissue section for my DAB stained sections?

10 November 2013 2,369 4 View

How can I detect activated rat alpha(IIb)beta(3) by flow cytometry?

In rat platelet samples

06 July 2013 278 1 View

Can someone share a standardised protocol for calpain (gelatin) zymography?

Using gelatin zymography for cardiac tissue homogenates.

06 July 2013 8,068 2 View

Does anyone have a standardised protocol for ex vivo rat heart model?

i wish to use an ex vivo rat heart model to identify the effect of er stress inducers following treatment of the animals with an antioxidant. I need help in the buffer and perfusion composition...

06 July 2013 4,068 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Mohammad M Farajollahi

Antibodies that are specified for wb may or may not suitable for immuprecipitation, but most of the antibodies that are suitable for ELISA can be used for immunoprecipitation. THAT IS because they can recognize the protein in the native form.

Jan Piwowarski

Small experiment with minimum of your antibodies, to check if you have at least traces of the protein are precypitated/co-precypitated. Hope, after the scale-up to have one kilogram of the protein;)

my suggestions only...

Keep fingers cross - jan

Kanika Jain

Thank you everyone for their answers...actually most of my antibodies are either WB specific or are suitable for IHC/ immunocytochemistry and since i am using a commercially availabe kit, i am not sure if i shld use my limited number of columns for the same...so if anyone has actually tried using the WB specific antibodies for IP..please guide me...