hi many company provide the buffer and lonza have nucleofection kits both  mouse and human. see i enclosed one company protocol

This protocol may work for HeLa, U2OS and 2091 fibroblasts.

http://www.med.nyu.edu/medicine/labs/gardnerlab/protocols/Electroporation%20Buffer%20for%20siRNA.pdf

may you can Benefit from the attached file

We've had good results with Optimem in place of the kits for various cell types.

Hi Mark,

Did you figure it out in the end? I am also looking for protocols to transfect vectors into various human cell lines using Amaxa nucleofector. Some of my cell lines are not in Amaxa database so I am looking for ways to do away with their kits.

Thanks.

You can check out the buffers listed in our paper: Efficient, Low-Cost Nucleofection of Passaged Chondrocytes. The buffer listed 1M worked on passaged chondrocytes. But the other listed buffers may work for other cell types.

Hi Justin,

I am not able to get access to your paper. Do you know of anywhere that is it publicly available at the moment?

Try Cytomix buffer:

van den Hoff, M.J.B., Moorman, A.F.M., and Lamers W.H. 1992. Electroporation in ‘intracellular’ buffer increases cell survival. Nucl. Acid. Res. 20 (11): 2902.

Chicaybam, L., Barcelos, C., Peixoto, B., Carneiro, M., Limia, C.G., Redondo, P., Lira, C., Paraguassú-Braga, F., Vasconcelos, Z.F.M.D., Barros, L., et al. (2016). An Efficient Electroporation Protocol for the Genetic Modification of Mammalian Cells. Front Bioeng Biotechnol 4, 99.

We described 7 different buffers for nucleofection of different mammalian cells.

How about a recipe for irreversible electroporation for anaerobic bacteria on a biofilm?

Iam trying to electroporate primary human cardiac fibroblast, can anybody suggest me a perfect buffer which I can use.

Article Phosphate-Buffered Saline-Based Nucleofection of Primary End...

Hello Everyone,

I am looking for a buffer and tips on electroporation of primary human bone marrow derived mesenchymal stromal cells. Any suggestions ? Thank you !

can cytomix be used in place of a lonza P3 buffer?