8 Questions 22 Answers 0 Followers
Questions related from Mark Bond
Does anyone have a recipe to make buffer for nucleofection of mammalian cells using a nucleofector machine?
24 May 2015 2,392 14 View
I have recently had problems getting good fluorescent images from gap tagged proteins in transferred cells. Basically I get fuzzy green background everywhere between the cells (I.e. The gaps). I...
27 January 2015 8,100 6 View
We recently got a new BioRad digital imaging western blot detection system. All is fine except that the software (ImageLab) can export images to TIFF but then refuses to import them again for...
02 July 2014 7,477 9 View
I am going to run some ChIP assays (qPCR based, not ChIP-seq) and am just wondering how important it is to include RNAse and Proteinase K treatment steps after elution from the beads and before...
25 May 2014 7,393 6 View
I would be interested in peoples opinion on the best way or presenting qPCR data. I often present data as relative expression, thats is relative to 1. Here a ten fold increase is plotted as a...
21 May 2014 2,854 23 View
I really need a plasmid to express the histone demethylase KDM6B/JMD3/Jumonji. Does anyone have one I could have please? I promise to acknowledge you on any paper we publish using it! Thanks.
26 March 2014 5,312 2 View
Has anyone tried crowdfunding their basic science research projects? I would be interested to hear about your experiences if you have.
28 January 2014 1,471 2 View
I have had some very faint signals from cells transfected with GFP tagged proteins and fixed for ten minutes in 4% paraformaldehyde. Is this fixation too harsh for GFP?
12 November 2013 1,180 51 View
