Mark Bond

6 Questions 22 Answers 0 Followers

Questions related from Mark Bond

Does anyone have a recipe for electroporation buffer?

Does anyone have a recipe to make buffer for nucleofection of mammalian cells using a nucleofector machine?

24 May 2015 2,378 14 View

Can you import TIFFs into BIORAD ImageLab software?

We recently got a new BioRad digital imaging western blot detection system. All is fine except that the software (ImageLab) can export images to TIFF but then refuses to import them again for...

02 July 2014 7,460 9 View

How important is RNAse and Proteinase K treatment in ChIP assays?

I am going to run some ChIP assays (qPCR based, not ChIP-seq) and am just wondering how important it is to include RNAse and Proteinase K treatment steps after elution from the beads and before...

25 May 2014 7,380 6 View

QPCR data - Positive/negative fold change or relative expression ratio?

I would be interested in peoples opinion on the best way or presenting qPCR data. I often present data as relative expression, thats is relative to 1. Here a ten fold increase is plotted as a...

21 May 2014 2,842 23 View

Does anyone have a mammalian expression plasmid for KDM6B/JMD3/Jumonji?

I really need a plasmid to express the histone demethylase KDM6B/JMD3/Jumonji. Does anyone have one I could have please? I promise to acknowledge you on any paper we publish using it! Thanks.

26 March 2014 5,299 2 View

What is the best fixation for fluorescence microscopy of GFP tagged proteins?

I have had some very faint signals from cells transfected with GFP tagged proteins and fixed for ten minutes in 4% paraformaldehyde. Is this fixation too harsh for GFP?

12 November 2013 1,168 51 View