Rachel Tam

2 Questions 3 Answers 0 Followers

Questions related from Rachel Tam

Do cells clump during incubation with Dynabeads?

Hi everyone. I am using Invitrogen CTS CD3/CD28 Dynabeads to simultaneously activate and isolate T cells from cryopreserved PBMCs. I thawed PBMCs in AIM-V medium, viability was 80% by trypan blue...

01 November 2016 6,245 4 View

Has anyone tried to reprogram cancer cells to iPSC?

I am trying to reprogram a mouse liver cancer cell line to iPSC. About 2 weeks after transfection I started seeing mouse ES-like colonies. I dissociated and replated them on new feeders hoping to...

04 May 2016 4,957 3 View