Rachel Tam

3 Questions 5 Answers 0 Followers

Questions related from Rachel Tam

Do cells clump during incubation with Dynabeads?

Hi everyone. I am using Invitrogen CTS CD3/CD28 Dynabeads to simultaneously activate and isolate T cells from cryopreserved PBMCs. I thawed PBMCs in AIM-V medium, viability was 80% by trypan blue...

01 November 2016 6,294 4 View

Has anyone tried to reprogram cancer cells to iPSC?

I am trying to reprogram a mouse liver cancer cell line to iPSC. About 2 weeks after transfection I started seeing mouse ES-like colonies. I dissociated and replated them on new feeders hoping to...

04 May 2016 5,021 3 View

Can I reprogram mouse cells without the use of feeder cells?

I am trying to reprogram a mouse cell line using transfection of yamanaka factors. During the iPSC induction phase (before emergence of colonies) can I keep the transfected cells simply on...

31 March 2016 9,798 4 View