Leonardo Chicaybam

3 Questions 14 Answers 0 Followers

Questions related from Leonardo Chicaybam

It is possible to sort cytokine producing cells, isolate DNA and perform PCR?

I would like to stain lymphocytes using an anti-IL2 antibody (for example), sort the positive cells using a flow cytometer and isolate DNA for PCR. Is this possible? Do you have some reference...

18 April 2016 6,576 2 View

What is the maximum number of PBMCs that can be frozen in one vial?

Assuming the use of FCS+10% DMSO as cryoprotectant and 1ml of final volume.

26 August 2013 8,639 6 View

Activation of human T lymphocytes with antibody-coated beads

I've searched the literature and found several papers that use Dynabeads M450 for the activation of T lymphocytes. However, there are two types of these beads: Epoxy and Tosylactivated. Which one...

10 April 2013 1,058 8 View