Leonardo Chicaybam

2 Questions 11 Answers 0 Followers

Questions related from Leonardo Chicaybam

It is possible to sort cytokine producing cells, isolate DNA and perform PCR?

I would like to stain lymphocytes using an anti-IL2 antibody (for example), sort the positive cells using a flow cytometer and isolate DNA for PCR. Is this possible? Do you have some reference...

18 April 2016 6,530 2 View

What is the maximum number of PBMCs that can be frozen in one vial?

Assuming the use of FCS+10% DMSO as cryoprotectant and 1ml of final volume.

26 August 2013 8,597 6 View