Does anyone have a good protocol for differentiating SH-SY5Y cells?

More Christa Maynard's questions See All

Alternative to microcapillary tube?

I'd like to run a quick pilot with synthetic volatile dispensers, assembled using a 2mL vial and piercing the septum with a microcapillary tube. I just discovered the lab is out of microcapillary...

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Mvabund package can't handle offsets?

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19 April 2021 7,131 0 View

If Moran's I is non-significant can I treat my samples as being independent?

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How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 4,269 1 View

How to remove inter-year variation from my assessment of seasonal variation?

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01 February 2021 4,234 5 View

Acid-resistant 96 well plates?

I'm doing a bioassay with sulfuric acid to be read in a spectrophotometer. Other than glass, are there less expensive options for 96 well plates that can handle sulfuric acid?

30 September 2020 9,565 2 View

Manual for MWG RoboGo!- automated pipetting station?

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21 August 2019 733 0 View

Teaching emotional intelligence in the literature classroom?

My university emphasises that courses in literature should teach transferable skills, like emotional intelligence. As an expert in literary studies of emotion, I conclude that teaching our...

28 July 2019 5,363 10 View

GDAL 2.4 fails to read Sentinel 1 GRD geoTiff reference projection. Fix?

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19 April 2019 6,512 1 View

MANOVA or Non-Parametric?

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11 April 2018 4,697 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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Weak DAPI staining after immunohistochemistry - how to improve?

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Are the GABAergic neurons electrophysiologically equal in all brain areas?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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Ravi Pottathil

Dear Christa

Try Retinoic acid see ref

http://www.ncbi.nlm.nih.gov/pubmed/21945098

Free Radic Biol Med. 2011 Dec 1;51(11):2007-17. doi: 10.1016/j.freeradbiomed.2011.08.030. Epub 2011 Sep 1.

Cristián Quintero

Dear Christa,

Besides retinoic acid, you also can try with cholesterol, retinoic acid + cholesterol, or retinoic acid followed by phorbol ester 12-O-tetradecanoylphorbol-13-acetate. See the references below. In the first one, the authors compare the effects of each treatment. In my case, I found the best results with RA+Cholesterol.

http://www.ncbi.nlm.nih.gov/pubmed/17540009

http://www.ncbi.nlm.nih.gov/pubmed/20497720