Which regression analyses to use?

Hi all, I am trying to analyse a set of data where the DV (called CWV) is continuous (it is the computed average score of three other variables), and has eight IVs (Ability, Prior, Field,...

21 November 2018 6,757 3 View

MANOVA or Non-Parametric?

I'm working on my dissertation proposal and I have to pre-determine the type of statistical analysis I'm going to run. I have 2 IVs (traditional gaming group vs. virtual gaming group), and I am...

11 April 2018 4,654 5 View

Protocol for lifting PMA-induced HL-60 cells?

I have made many attempts to raise adherent macrophage-like HL-60 cells in order to run phagocytosis assays and flow, but I cannot seem to lift them without killing or damaging the vast majority...

07 March 2016 4,500 4 View

Is anyone familiar with stable cell line generation?

I'm getting rather desperate now with continually failing to obtain stable cell lines. My cells just all die when i add selection agent, even though i tested with a kill curve at a range of cell...

13 October 2014 2,619 7 View

Does anyone have a good protocol for differentiating SH-SY5Y cells?

I want to differentiate the cells mainly so that they stop dividing, but also so that they acquire more 'neuronal like' characteristics since I'm working on neurodegenerative diseases.

15 May 2014 9,534 2 View

Emotional Intelligence and Fear Management

We live in an age of fear. The hysterical responses to the coronavirus corroborate a whole range of additional fears: fear of the implications of the Fourth Industrial Revolution, fear of growing...

01 January 1970 1,826 12 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

Nuances in the digital twin concept. Opinions?

Hello everyone, I am currently working on the state of the art of the digital twin concept, and after seeing the categorization made by Kritzinger, W. et al. (2018) [Digital Twin in...

14 February 2021 2,107 3 View

What Research is yet left in the field of Automation and Unemployment?

I have been through a number of journals and Research Papers. I wanted to get an idea as to what new areas are still left to be researched in "Will Automation lead to unemployment or it will lead...

09 February 2021 9,332 4 View