Does anyone have a detailed protocol for the isolation of microglial cells from mouse brain using percoll?

05 May 2012 1 9K Report

Microglia isolation using percoll

Here is a protocol for you to try. There is a reference at the bottom of the protocol that should give more information.

Badges
Science topic

More Ismail Amr El-Shimy's questions See All

What are the recommended software tools for simulating the converter-driven dynamics?

For more details about the converter interfaced generation and their impacts on power system stability, you may read Definition and Classification of Power System Stability Revi... What are the...

15 December 2020 6,644 2 View

How to measure the eddy currents?

An eddy current is a well known reaction in electromagnetism. They exist in the ferromagnetic core of various static and moving electromagnetic induction based machines, for example, transformers...

10 November 2018 9,979 4 View

Is it possible to reverse the mass to energy relation?

If you have the amount of energy resulting from transforming a mass m into energy (i.e E = m C^2), can an equivalent mass be created?

06 July 2018 9,384 28 View

Can cetuximab recognize mouse EGFR?

I was looking for an antibody that can block the ligand binding domain of mouse EGFR. Can I use Cetuximab or is it exclusively human-specific?

13 June 2018 586 9 View

Can cetuximab recognize mouse EGFR?

I was looking for an antibody that can block the ligand binding domain of mouse EGFR. Can I use Cetuximab or is it exclusively human-specific?

05 June 2018 1,035 6 View

I need a detailed and standardised method for electromechanical model scaling of electric power systems, any help?

It is known that mega-scale systems can be modeled in laboratories using scaled modeling approach. As a result a miniature system which is equivalent to the original system can be created. On the...

04 May 2018 2,995 0 View

How to deal with unavoidable haters in a rational way?

I guess that anyone faces at least unjustified hate from a hater in his/her life. IMHO, haters are weak, and psychologically ill people. Probably, the best way to deal with these personalities is...

24 April 2018 2,575 3 View

How to determine the permissible step load change on a battery?

I would like to determine (through measurements and/or digital simulation) the permissible step (or sudden) load change on a battery. The main target is to satisfy the safe limits such as...

21 April 2018 3,125 1 View

Is there a specific web resource that provides updated set of keywords in electric power and energy?

The keywords of a specific discipline are very important in identifying the research scopes, directions, and trends of research. If you know a specific website that provides updated keywords of...

11 April 2018 6,827 6 View

Why do you want to publish your research?

Please state your reasons to publish your research.

03 April 2018 6,166 15 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View