Microglia isolation using percoll
Here is a protocol for you to try. There is a reference at the bottom of the protocol that should give more information.
For more details about the converter interfaced generation and their impacts on power system stability, you may read Definition and Classification of Power System Stability Revi... What are the...
15 December 2020 6,426 2 View
An eddy current is a well known reaction in electromagnetism. They exist in the ferromagnetic core of various static and moving electromagnetic induction based machines, for example, transformers...
10 November 2018 9,610 4 View
If you have the amount of energy resulting from transforming a mass m into energy (i.e E = m C^2), can an equivalent mass be created?
06 July 2018 9,149 28 View
I was looking for an antibody that can block the ligand binding domain of mouse EGFR. Can I use Cetuximab or is it exclusively human-specific?
05 June 2018 846 6 View
It is known that mega-scale systems can be modeled in laboratories using scaled modeling approach. As a result a miniature system which is equivalent to the original system can be created. On the...
04 May 2018 2,822 0 View
I would like to determine (through measurements and/or digital simulation) the permissible step (or sudden) load change on a battery. The main target is to satisfy the safe limits such as...
21 April 2018 2,920 1 View
Please state your reasons to publish your research.
03 April 2018 6,001 15 View
Dear young researchers, The Publons Academy is a practical peer review training course for early career researchers developed together with expert academics and editors to teach you the core...
03 April 2018 5,038 4 View
03 April 2018 7,082 1 View
I guess that anyone faces at least unjustified hate from a hater in his/her life. IMHO, haters are weak, and psychologically ill people. Probably, the best way to deal with these personalities is...
03 April 2018 3,713 4 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View