Does anyone has experience with the IEC-18 cell line?

More René Renato Balandrán-Quintana's questions See All

How to perform GEE (Generalized estimating equations) analysis in SPSS to compare injuries?

I am working on a project that requires the usage of GEE for statistical analysis. The idea is to compare to groups that either use an intervention or not and see whether injuries differ between...

28 January 2024 8,019 1 View

Are there historical studies on the trade and/or circulation of automata in Latin America, during the 19th and 20th centuries?

Source of image: Jaquet Droz automata.

17 May 2023 9,127 1 View

How to calculate standard deviation calculation for percentage of change?

I´m performing a meta-analysis and some studies report continuous data as percentage of change (pre vs. post) but does not report standard deviation from this percentage of change. I´ve been...

04 May 2023 2,326 3 View

ANOVA with percentage data in SPSS?

I have a set of data from three groups across 3 time points (2 groups are subjected to an intervention and the third acts as control). I want to evaluate intra and inter-group differences between...

08 March 2023 6,758 3 View

How do I make a 100nM final concentration of Ouabain?

Hello, I am doing an Ouabain resistance mutation assay and I have to use a concentration of 100nM. I'm struggling with determining how many grams I should use for my solution. Would greatly...

26 September 2021 4,682 1 View

I am 2D differentiating GABAergic neurons from hiPSCs. Is there any approx time correspondence with human embryonic differentiation?

I am following this protocol (EB mediated version)Directed differentiation of forebrain GABA interneurons from... which gives me Gad1 positive neuron networks around day 45. I am trying to...

09 July 2020 4,834 0 View

Trying to choose between a LM or a GLM (family=negative binomial)?

Hi! I have data on captures per year (n=13) and I want to analyze the relation with years (is it increasing, decreasing or staying the same with time). Total captures ~ year Because it is...

23 May 2020 6,152 3 View

What would be the best way to deal with college dropout?

I would like to be able to predict and determine the causes of college student dropout.

14 May 2020 6,939 1 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

20 January 2019 2,120 3 View

How I remove the link for a paper?

I recently uploaded a public preprint of my work in reserchgate. I have now made it private, howevere, google scholar still has the link to the full pdf paper. Is it possible to remove the link or...

15 January 2019 568 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View