Does anybody provide me a protocol for coagulase protein extraction from staph aureus?

11 November 2016 1 7K Report

I am trying to isolate coagulase from S. aureus through ammunium sulfate but I did not get any success when i run SDS PAGE. Does any body provide me protocol for it?

Thank you!

Adam B Shapiro

This paper uses a different method, and has references to other methods:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC348089/pdf/iai00144-0240.pdf

Badges
Science method

More Bushra Bilal's questions See All

I need a protocol for the extraction of proteins from plant leaves. Can any body help me?

I have tried to extract the proteins from the plant eaves through phenol acetone method and I got the white pellet at the end of experiment but when I run the SDS PAGE I got smear and very little...

05 June 2016 4,501 3 View

What should be the maximum cycle number in qPCR?

I wanted to know that I am putting 50 cycles in my qPCR program because I obtain my plateau after 40 cycles. Is it Ok or I do need to minimize it upto 40 cycles?

02 March 2016 4,439 7 View

What are the housekeeping genes for C.elegans qPCR?

I am working with C elegans N2 strain. What are the reference genes for them to carry out qPCR?

08 September 2015 4,513 5 View

How to extract protein from SDS-PAGE gel?

Does anyone know how a protein band can be extracted from SDS-PAGE gel without using any commercial kit?The gel is stained by coomassie brilliant blue.

01 February 2015 3,378 3 View

How can I analyse peptides on SDS PAGE?

I have GFC fractions containing peptides less than 2 KDa so what should be the conditions and gel composition to analyse peptides on SDS PAGE?

01 February 2015 3,701 3 View

Does anyone know how to run SDS PAGE for glycoroteins?

I have been trying to purify glycoroteins but they are fail to appear on SDS PAGE because they are highly coated with sugars. How can i Stain them through manual method?

31 December 2014 9,091 9 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View