What are the housekeeping genes for C.elegans qPCR?

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Luca Cirillo

A friend of mine used 28S rRNA but I do not know if it worked,

Daniel L. Starnes

We have used gpi-1, B-actin also works fairly well. Just need to make sure that none of your treatments alter the expression.

Bushra Bilal

I have to check the effect of some proteins on the expression of these house keeping genes.

We have switched to using Y45F10D.4. Its been great reference genes. And here is a paper to go with it.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3305280/

Hi Daniel

Its is good paper and i have tried these primer for my experiment but now i have to design the primers related to the reproductive system of C elegans.