Bushra Bilal

7 Questions 14 Answers 0 Followers

Questions related from Bushra Bilal

Does anybody provide me a protocol for coagulase protein extraction from staph aureus?

I am trying to isolate coagulase from S. aureus through ammunium sulfate but I did not get any success when i run SDS PAGE. Does any body provide me protocol for it? Thank you!

11 November 2016 7,175 1 View

I need a protocol for the extraction of proteins from plant leaves. Can any body help me?

I have tried to extract the proteins from the plant eaves through phenol acetone method and I got the white pellet at the end of experiment but when I run the SDS PAGE I got smear and very little...

06 June 2016 4,479 3 View

What should be the maximum cycle number in qPCR?

I wanted to know that I am putting 50 cycles in my qPCR program because I obtain my plateau after 40 cycles. Is it Ok or I do need to minimize it upto 40 cycles?

03 March 2016 4,420 7 View

What are the housekeeping genes for C.elegans qPCR?

I am working with C elegans N2 strain. What are the reference genes for them to carry out qPCR?

09 September 2015 4,483 5 View

How can I analyse peptides on SDS PAGE?

I have GFC fractions containing peptides less than 2 KDa so what should be the conditions and gel composition to analyse peptides on SDS PAGE?

02 February 2015 3,675 3 View

How to extract protein from SDS-PAGE gel?

Does anyone know how a protein band can be extracted from SDS-PAGE gel without using any commercial kit?The gel is stained by coomassie brilliant blue.

02 February 2015 3,346 3 View

Does anyone know how to run SDS PAGE for glycoroteins?

I have been trying to purify glycoroteins but they are fail to appear on SDS PAGE because they are highly coated with sugars. How can i Stain them through manual method?

01 January 2015 9,070 9 View