How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Possible problem for showing 0.2 sun intensity instead of 1 sun intensity in AAA class Newport solar simulator?

We have a AAA class Newport solar simulator. We changed the Xenon Arc Lamp recently (XBO450W, Make:OSRAM) as the lifetime got over. However, the intensity is coming to be only 0.2 sun instead of 1...

14 February 2021 9,998 3 View

Any reference paper with detailing on how to hybradize Q-Learning algorithm with Genetic Algorithm, especially for a variable optimization problem?

I am planning to hybradize Q-Learning algorthm with Genetic algorithm at the population initialization stage, crossover stage and mutation stage. The problem under consideration is a...

07 February 2021 3,123 8 View

How to compare expected and counted values statistically with different p values for each genotype?

Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...

03 February 2021 6,408 2 View

What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

What causes defect peak broadening?

Say we observe yellow and blue luminescence in GaN, and these defect peaks are always broader than the BE peak. What factors contribute to defect peak broadening? Please help me list out such...

25 January 2021 1,733 3 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Are Signal Peptide and Transit Peptide different? And what are their functions?

I want to learn about mutation in transit peptide. Can mutation in transit peptide affect the function of the protein?

20 January 2021 396 3 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View