Chandra Ramakrishnan

4 Questions 9 Answers 0 Followers

Questions related from Chandra Ramakrishnan

How can a phosphoprotein have a multiplicity higher than 1, but only one S/T/Y labelled with a high probability?

I have performed label-free phosphoproteomics and analysed the data with MaxQuant. Now I get phosphopeptides marked being doubly phsphorylated, but only one amino acid has a high probability of...

26 February 2020 5,161 6 View

Purified nanobody works in IFA, but not in immunoprecipications. Has anybody else had this experience?

Phage-display is done using a Triton X-100 cell extract to select our clones. Nanobodies are then produced in the periplasm of E. coli, extracted using B-Per and purified using NiNTA beads...

28 August 2019 6,926 6 View

Does anybody know what the length of a canonical polypyrimidine tract is in Toxoplasma gondii?

I am currently looking into intron and if mutations at the 3' end of an intron are likely to cause a splicing defect. I have not way of experimentally validating this.

26 February 2018 1,466 3 View

Does anybody know how to predict branch points in intron in Toxoplasma gondii or in any Apicomplexa?

I am trying to map a mutation in an intron to see if it may cause splicing defects. I cannot validate this experimentally.

26 February 2018 6,248 0 View