Does anybody know universal promoter which was used for gene expression in fungi (basidiomycets)?

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Yuan-Yeu Yau

Hi Mohsen,

I have seen some papers published using 35S promoter to drive a gene in some fungi. If you cannot find documentation about transgenesis on basidimycetes (your experiment material), you can first try some transient assay using 35S promoter and a GFP gene (35S::gfp) to see if the 35S promoter can drive the gfp gene and glow in your fungus tissue. You can try a quick bombardment experiment. You can also use other screenable genes, such as gus gene.

Mohsen Ashrafi

special thanks for your answer Yuan-Yeu Yau but efficiency of 35s is variable in basidiomycete. we used this promoter for transformation of agaricus bisporus and it has been worked. we also used gpdII homologous promoter and it has been worked in mentioned fungi. we must try these promoters.

Did you use an enhanced 35S promoter (with double 'enhancer' in it) or just a regular 35S promoter? An enhanced 35S promoter usually give a stronger expression level.

we used pCAMBIA1304 for transformation of Agaricus bisporus. this plasmid has double 35s promoter for expression of selection marker gene and also has regular 35s promoter for expression of integrated GUS-GFP. First promoter have been working good but not perfect but we couldn't detect any GUS-GFP RNA, after transformation. probably we use double 35s promoter for transformation of other basidiomycetes.

thanks Yuan

Gurdee Kaur

I tried R. solani fungal transformation with plasmid containing 35s promoter. but it did not work. Please suggest some other promoters for stable transformation!

Thanks