Which statistical test should I use for my data analysis?

Hi, I want to statistically analyse my metabolomics data (18 metabolites). I have two species and four levels of drought. I analysed each species individually by CRD and for mean comparison I used...

01 February 2018 3,866 5 View

How much time is needed to remove methanol from 2 ml of a mixture?

Dear researchers, I want to remove methanol from 2 ml mixture of 50/50 water/methanol (as a solvent for primary metabolite extraction) and it is recommended to me to use speed vac instrument. I am...

09 October 2017 5,221 16 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

01 February 2017 9,722 4 View

Do DEPC treated water before autoclaving make foam?

09 October 2016 1,327 6 View

How can I draw a plot with custom Y-axis in R?

07 August 2016 2,520 6 View

Can I quantify rubisco and rubisco activase content only by SDS-PAGE?

In my thesis I will need to compare rubisco and rubisco activase content at protein level. Can I use SDS-PAGE for comparison of rubisco and rubisco activase content in two samples or I must use...

02 March 2015 6,123 10 View

Does anybody know how to draw a metabolite network and extract important metabolites from that?

10 November 2014 395 7 View

Does anybody know universal promoter which was used for gene expression in fungi (basidiomycets)?

Thanks

08 September 2014 3,369 5 View

Why is realtime PCR more sensitive than PCR?

Many articles said that Realtime PCR can detect as less amount of DNA or RNA as PCR. How do you explain this?

05 June 2014 4,228 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View