Which statistical test should I use for my data analysis?

Hi, I want to statistically analyse my metabolomics data (18 metabolites). I have two species and four levels of drought. I analysed each species individually by CRD and for mean comparison I used...

01 February 2018 3,866 5 View

How much time is needed to remove methanol from 2 ml of a mixture?

Dear researchers, I want to remove methanol from 2 ml mixture of 50/50 water/methanol (as a solvent for primary metabolite extraction) and it is recommended to me to use speed vac instrument. I am...

09 October 2017 5,221 16 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

01 February 2017 9,722 4 View

Do DEPC treated water before autoclaving make foam?

09 October 2016 1,327 6 View

How can I draw a plot with custom Y-axis in R?

07 August 2016 2,520 6 View

Can I quantify rubisco and rubisco activase content only by SDS-PAGE?

In my thesis I will need to compare rubisco and rubisco activase content at protein level. Can I use SDS-PAGE for comparison of rubisco and rubisco activase content in two samples or I must use...

02 March 2015 6,123 10 View

Does anybody know how to draw a metabolite network and extract important metabolites from that?

10 November 2014 395 7 View

Does anybody know universal promoter which was used for gene expression in fungi (basidiomycets)?

Thanks

08 September 2014 3,369 5 View

What is the maximum length of DNA which can be transformed by Agrobacterium tumefaciens?

08 September 2014 6,838 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View