Mohsen Ashrafi

10 Questions 74 Answers 0 Followers

Questions related from Mohsen Ashrafi

Which statistical test should I use for my data analysis?

Hi, I want to statistically analyse my metabolomics data (18 metabolites). I have two species and four levels of drought. I analysed each species individually by CRD and for mean comparison I used...

02 February 2018 3,957 5 View

How much time is needed to remove methanol from 2 ml of a mixture?

Dear researchers, I want to remove methanol from 2 ml mixture of 50/50 water/methanol (as a solvent for primary metabolite extraction) and it is recommended to me to use speed vac instrument. I am...

10 October 2017 5,314 16 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

02 February 2017 9,813 4 View

Do DEPC treated water before autoclaving make foam?

Hi every body I added DEPC to water and then shake it vigorously and I saw that it mad foam. After autoclaving I did not see any foam. Is it natural? Best regards

10 October 2016 1,419 6 View

How can I draw a plot with custom Y-axis in R?

Hi every body I want to draw a bisection Y-axis plot which scaled from 0 to 100. At first section (1 to 30) it would be ranged by 2 (2,4,6,...,30)and after that it would be ranged by 10...

08 August 2016 2,614 6 View

Can I quantify rubisco and rubisco activase content only by SDS-PAGE?

In my thesis I will need to compare rubisco and rubisco activase content at protein level. Can I use SDS-PAGE for comparison of rubisco and rubisco activase content in two samples or I must use...

03 March 2015 6,276 10 View

Does anybody know how to draw a metabolite network and extract important metabolites from that?

Hi, I want to draw a metabolite network and then identify important metabolites from that network. I will use Cytoscape but I don't know how to draw the network and how to identify important...

11 November 2014 482 7 View

What is the maximum length of DNA which can be transformed by Agrobacterium tumefaciens?

Hi everybody. Does anybody know the maximum length of DNA (between left border and right border of plasmid) which can be transformed by Agrobacterium tumefaciens? thanks

09 September 2014 6,930 8 View

Does anybody know universal promoter which was used for gene expression in fungi (basidiomycets)?

Thanks

09 September 2014 3,458 5 View

Why is realtime PCR more sensitive than PCR?

Many articles said that Realtime PCR can detect as less amount of DNA or RNA as PCR. How do you explain this?

06 June 2014 4,328 3 View