How much time is needed to remove methanol from 2 ml of a mixture?

Dear researchers, I want to remove methanol from 2 ml mixture of 50/50 water/methanol (as a solvent for primary metabolite extraction) and it is recommended to me to use speed vac instrument. I am...

09 October 2017 5,221 16 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

01 February 2017 9,722 4 View

Do DEPC treated water before autoclaving make foam?

09 October 2016 1,327 6 View

How can I draw a plot with custom Y-axis in R?

07 August 2016 2,520 6 View

Can I quantify rubisco and rubisco activase content only by SDS-PAGE?

In my thesis I will need to compare rubisco and rubisco activase content at protein level. Can I use SDS-PAGE for comparison of rubisco and rubisco activase content in two samples or I must use...

02 March 2015 6,123 10 View

Does anybody know how to draw a metabolite network and extract important metabolites from that?

10 November 2014 395 7 View

Does anybody know universal promoter which was used for gene expression in fungi (basidiomycets)?

Thanks

08 September 2014 3,369 5 View

What is the maximum length of DNA which can be transformed by Agrobacterium tumefaciens?

08 September 2014 6,838 8 View

Why is realtime PCR more sensitive than PCR?

Many articles said that Realtime PCR can detect as less amount of DNA or RNA as PCR. How do you explain this?

05 June 2014 4,228 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View