Do they deteriorate with time? And is there any way of getting them more clear?
Cloudy, turbid plaques can indicate a temperate phage. The cells that incorporate the virus as prophage (lysogeny) continue to replicate, causing the turbid plaque. This is a stable characteristic of temperate phage.
I would appreciate your feedback on this issue. I am interested in evaluating the presence and subsequently quantifying AI-2 molecules in some bacterial cultures that I am working with. So, I...
19 March 2019 5,693 2 View
I have a 30 mm thick plate AA7050 alloy and I want to reduce the residual stresses without altering the microstructure; so I cannot heat up the specimen. What techniques are out there? Is it...
19 February 2018 9,984 1 View
Could you tell me please, how much is the specific weight of chopped raw garlic and chopped raw onion? Thank you.
04 March 2016 8,009 1 View
I was just curious as to find the definition and detailed explanation on the formation of Luders bands. And I found the below caption on Wikipedia. Is the information correct? Extensive research...
01 January 1970 5,195 3 View
Which species is entomopathogenic nematodes is effective against: - Frankliniella occidentalis - Thrips tabacci What are the recommended dose rates?
01 January 1970 6,594 3 View
My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....
08 February 2021 5,965 1 View
Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...
27 January 2021 5,767 3 View
Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...
24 January 2021 2,767 6 View
I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...
22 January 2021 8,612 3 View
Bacteriophages very effective against infections. Many of them such as anti-Staphylococcus or anti-Streptococcal widely used in practical medicine. Some bacteriophages can be oncolytic and...
03 January 2021 4,769 1 View
Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...
18 December 2020 9,931 2 View
Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...
13 December 2020 9,167 9 View
I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...
25 November 2020 858 2 View
I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....
15 November 2020 7,488 4 View
i went through many literature to find out the exact viral vector but it was generally mentioned m13 vector in the papers not specific about the name, can anyone suggest me which m13 vector can...
18 October 2020 2,644 1 View