Panos G. Kalatzis

5 Questions 3 Answers 0 Followers

Questions related from Panos G. Kalatzis

Hello! Which would be the best methods to quantify quorum sensing molecules, particularly AI-2?

I would appreciate your feedback on this issue. I am interested in evaluating the presence and subsequently quantifying AI-2 molecules in some bacterial cultures that I am working with. So, I...

20 March 2019 5,827 2 View

Hi! I would like to ask, in your opinion which are the most effective ways to delete a prophage from a bacterial genome??

If a bacterial genome is sequenced and we know that it contains a prophage, whose presence has been also verified by induction in the lab, which would be the best ways to specifically delete the...

12 February 2018 7,831 3 View

I have just got a vial of Mitomycin C from Sigma (2mg mitomycin c and 48mg NaCl). What method do you use for this?

According to the manual, water is just fine to dissolve it. I want to use mitomycin c for examining bacterial strains for prophages... Thanks!!

23 March 2015 4,039 1 View

I am trying to extract DNA from bacteriophages using a phenol - chloroform protocol. Do you have any ideas?

The problem is that in the gel the DNA bands are formed very very low. It is like fragmented DNA. I thought that the problem may be the precipitation of these phages with PEG 10% because another...

27 November 2013 6,710 4 View

Does anybody know the meaning of obscure phages plaques?

Do they deteriorate with time? And is there any way of getting them more clear?

17 December 2012 4,079 1 View