I would appreciate your feedback on this issue. I am interested in evaluating the presence and subsequently quantifying AI-2 molecules in some bacterial cultures that I am working with. So, I would like to ask from those of you who are familiar with this topic to bring some insights of different approaches. For example, are there any culture media that I could use to detect it, is qPCR for LuxS enough, would HPLC work, or have you done any other techniques that gave reliable results?? Thank you in advance for your time!
Bac Nguyen
I think the best method is HPLC-MS-MS. I tried to quantify C4 and C12-HSL of Pseudomonas aeruginosa and it worked w
1 votes
1 thanks
Dawid Gmiter
The strain Vibrio harveyi BB170 as an AI-2 reporter strain could be useful.
0 votes
0 thanks
- Badges
- Science method
More Panos G. Kalatzis's questions See All
Similar questions and discussions