Want to determine genotoxicity in fishes.
Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.
14 July 2024 3,120 7 View
I am interested in understanding the effectiveness of zinc oxide (ZnO) for water purification or treatment. I would like to know about its specific mechanisms and applications in addressing water...
14 June 2024 5,389 1 View
Give a concrete example to prove this
06 June 2024 3,630 0 View
How do we check the strength of a mediation hypothesis (calculated by Hayes Process Macro) by "Variance Accounted for Technique"?
11 May 2024 3,044 0 View
why electron and hole thermal velocities are same for all materials in scaps simulation, why
27 April 2024 2,906 1 View
HIV uncontrolled in south Punjab and Sindh
04 February 2024 1,891 2 View
I have done miRNA sequencing and there are 464 novel miRNAs. How is it to show novel miRNAs on heat map, volcano plot along with known miRNAs? Secondly, I also want to know how to go ahead with...
01 February 2024 1,383 4 View
I have attached my CAE file I am a beginner with ABAQUS and trying to do cyclic analysis but not getting RF Graph to make an Hestrisis Diagram. and also don't know how to perform Analysis Note You...
22 January 2024 9,822 2 View
I'm trying to use CDP model but i only have ultimate strength of concrete how should i create stress strain data sheet
10 January 2024 6,495 7 View
Cement 863 kg/m3 Sand 880 kg/m3 Silica Fume 215 kg/m3 Fly ash 159 kg/m3 HRWR (Sika Viscocrete 20HE) 0.014% Water 0.18
27 December 2023 2,131 8 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View