Mehjabeen Javed

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Questions related from Mehjabeen Javed

I have done miRNA sequencing and there are 464 novel miRNAs. How is it to show novel miRNAs on heat map, volcano plot along with known miRNAs?

I have done miRNA sequencing and there are 464 novel miRNAs. How is it to show novel miRNAs on heat map, volcano plot along with known miRNAs? Secondly, I also want to know how to go ahead with...

02 February 2024 1,360 4 View

RNA concentration in a sample was 5-16µg/µL, 260/280 & 260/230 ratios were also good. However samples fail QC result by QUBIT FLUOROMETER & RIN 1-1.8?

Recently, I did an experiment where RNA was isolated by TRIZOl reagent. Finally the concentration of RNA check by nanodrop was around 5-16 microgram/microliter. Their 260/280 ratios were in the...

03 July 2023 2,822 13 View

I want to do RTPCR for some biomarkers from the serum. But the RBCs get lysed so it turns slightly RED. Does it affect GENE EXPRESSION RESULTS ?

I want to do RTPCR for some biomarkers from the serum. But unfortunately, the RBCs get lysed due to this it turns slightly RED. Does it affect the gene expression or RTPCR RESULTS?

28 January 2023 6,279 3 View

Effect on MARK4 activity and microtubule of alzheimer mice brain?

I have to determine the effect of some phytoconstituent on MARK4 and microtubule of neuron. How can I do it. I mean do I have to first extract the MARK4 from hippocampus and cortex by RT PCR and...

13 October 2020 824 1 View

Can someone provide protease, amylase and lipase estimation protocol in fishes ?Could anyone share it with me?

I want to check the levels of digestive enzymes of fishes in general, therefore I am searching for spectrophotometric analysis protocol.

10 October 2015 717 4 View

Why is the estimated daily intake is more than the reference dose, but the target hazard quotient (THQ) is still less than one?

I am working on risk assessment of heavy metals and the results are such that Estimated Daily Intake (EDI) is many folds greater than respective reference dose (RfD), but the THQ is less than...

17 December 2014 3,113 3 View

What is the latest FAO/WHO permissible limits for these metals (Cr, Mn, Fe, Co, Ni, Cu and Zn) in foods ?

Currently, I am working on aquatic toxicology, particularly heavy metals. Therefore I want to know the latest maximum permissible limits of these heavy metals (Cr, Mn, Fe, Co, Ni, Cu and Zn) in...

22 November 2014 3,659 17 View

What is the relation between bioavailability and accumulation of heavy metals to fish?

I have worked out the bio-availability of Cu, Ni, Fe, Co, Mn, Cr and Zn in 3 different water bodies and their accumulation in the model fish channa punctatus. I don't get any trend in relation to...

28 April 2014 1,662 2 View

Is there anyone working on fish brain?

I am working on toxicity to fish brain with different toxicants. Unfortunately literature is not available, if some one could help me getting relevant data related to the topic.

23 April 2014 244 1 View

How is it that rbc production induces hypoxia in fish?

I am working on hematotoxicity in fish with heavy metals. In one of the papers I read, it said that if haemoglobin and haematocrit increased, then it causes hypoxia in fish. I think that an...

13 January 2014 508 1 View

I need to convert enzyme unit (IU/L) to mg/g, how can I do that?

I determine the enzyme activity in fish liver and muscle by using the diagnostic kit. This mentioned in kit that after calculation the activity is in IU/L, since the kit is designed for serum...

09 December 2013 3,491 14 View

I want to study the serum lipid profile of fish after inducing it with heavy metals. Could anybody tell me - is it feasible to do so?

I want to work out the serum lipid profile of fish after affecting with heavy metals. Is it fine to do so? And only serum lipid profile is enough? What will I derive from it?

09 December 2013 4,566 10 View

Can anyone tell me if making homogenate in phosphate buffer (50mM, ph 7.2) is enough to determine the lipid profile?

I am determining the lipid profile of fish liver and muscle by simply making the homogenate in phosphate buffer (50mM, ph 7.2) centrifuge and then proceed according to the protocol given in the...

20 November 2013 8,764 2 View

Could anybody tell me how can I determine a lipid profile from a fish liver and muscle?

I want to determine the lipid profile of fish liver and muscle but I am not getting any suitable and simple protocol. Could anybody suggest some simple methodology?

20 November 2013 5,818 4 View

Could someone help me work out the target hazard quotient?

I am working on heavy metal and need to calculate THQ. Would any body help me to calculate and also tell me what is what in this formula THQ = E F x F D xDIM ∕ RfDxWxT. Is any value constant in...

18 November 2013 1,116 6 View

Why is Fe concentration in polluted water highest among Cu, Ni, Fe, Co, Mn, Cr and Zn?

I estimate the heavy metals in 3 water bodies polluted by 3 different sources i) polluted by thermal power plant ii) polluted by sewage and agricultural runoff iii) polluted by sugar mill...

24 September 2013 5,401 3 View

Why does lipidogenesis occur in fish liver when it is inhabiting natural water body and is polluted?

I want to know whether the cause of lipidogenesis in Channa punctatus is heavy metals which are present beyond permissible limits in the water body?

04 September 2013 7,083 5 View

Is it feasible to determine levels of GPT, GOT and total protein from the dried fish tissues?

I want to know whether I can determine the enzymes GPT, GOT (by GOT and GPT KIT) and total protein (by Lowry method) from fish liver and muscle dried at 60 C, or do I just use fresh tissues?

02 September 2013 5,786 6 View

Could anybody tell me the cost effective and easy method to remove heavy metals from large natural water bodies?

I want to know if there is any cheap and easy method for us to remove heavy metals from large natural bodies of water? We will do the biosorption and adsorption experiments in the lab.

23 August 2013 9,476 53 View

I did rotary microtomy on gill section of fish channa punctatus but I'm not getting what deformities are present in it.

I did microtomy by paraffin embedding and the section I get is observed under Nikon eclipse 80i microscope at 40x. I attached the figure in the hope that someone could tell me if the deformities...

20 August 2013 7,733 3 View

Could anybody tell me what does it mean when you obtain a negative value after analysis by Atomic Absorption Analysis?

I am working on atomic absorption spectrophotometer, I analyzed the sample for Cd but the reading I obtained after analysis is NEGATIVE? What does this mean, is it absent in the sample or below...

02 August 2013 9,287 3 View

Could the metal pollution index (MPI) and bioaccumulation factor (BAF) be used simultaneously?

I have calculated MPI and BAF for the same fish for Cu, Ni, Fe, Co, Mn and Zn. The result obtained by MPI follows the trend liver>kidney>gills>integument>muscle. While from the BAF was...

05 June 2013 5,854 14 View

Can I use the contamination factor and ecological risk factor for water polluted by heavy metals?

I am working on heavy metal pollution in water bodies and particularly concerned with surface water and the fauna. Therefore, I want to know if these two factors will be applicable in water...

19 April 2013 7,703 16 View

Could anybody give me the possible reasons why Fe accumulation is higher than other minerals in fish?

I am working on toxicity of Fe, Zn, Cu, and Cr from three different sites on a single fish species. From each site the accumulation of Fe is higher than the other minerals and also its presence in...

18 April 2013 2,336 17 View

Is it correct to work out Bioconcentration factor and Metal pollution index at the same time in single species collected from a single site?

I am working on the accumulation of seven metals in a single species collected from a single site. I want to use both BCF and MPI, can anyone tell me if it is ok to do so? Also, can anyone tell me...

15 April 2013 8,726 6 View

Is it correct to calculate metal pollution index in different organs of single species collected from one site in one season?

I am working on Channa punctatus collected from one site and now want to calculate the metal pollution index (Cu, Ni, Fe, Co), so please tell me is it feasible to calculate MPI in different organs...

12 April 2013 6,625 16 View

Does anybody know how the aquatic toxicity index is calculated?

I am working on aquatic toxicology. My work purely involves field study. Therefore, I want to know what this index actually is and how it can be calculated?

04 April 2013 4,799 8 View

Primer designing for miRNA

I am working on different miRNA expressions in Rattus norvegicus. Could any one guide me where and how can I designed the primer for any specific miRNA? Is it the NCBI platform for this too? What...

01 January 1970 6,659 2 View

IL8 primer for Rattus norvegicus

I am working on proinflammatory cytokines IL6 and IL8 in chemically induced Rattus norvegicus. I have designed the primer for IL6 but unable to do it for IL8, because I do not find any data for...

01 January 1970 8,254 0 View