Does anybody here have a good link for tutorials in using vector NTI software for sequence analysis?

More Ibrahim Thabet's questions See All

Open Reading Frame (ORF)/Vector NTI?

How do I decide which is the right ORF (Open Reading Frame) for the translation using vector NTI software?

03 April 2017 5,910 5 View

I am preparing my manuscript for publication. Any recommendations for free publishing, as funding is very limited?

My paper is on the influenza virus. Do you know of any free peer reviewed journals in the area of Virology where I could publish?

11 December 2013 7,255 4 View

What is the importance of standard curve for RT-qPCR, I did standard curve for M gene H5N9 (known PFU), compared it to H5N1 M gene, is this correct?

10 November 2013 3,383 9 View

How can you solve a problem of non specific bands at RT-PCR, you expect at 700 bp and you get clear band at 500 - can anybody help?

08 September 2013 512 21 View

Does anybody have experience with using Roch PCR kits?

I do not know why my samples give good results with TAKARA protocol and not for Roch protocol. Roch High fidelity enzyme.

07 August 2013 8,740 0 View

Does anybody know what the mechanism of action of RLP lysis buffer of Qiagen kits is?

By which mechanism do they extract RNA, and why is RNA in this lysate very stable ?

07 August 2013 450 15 View

I want to prepare formalin fixed blocks for immunohistochemistry, samples preserved in formalin 10 % from 3 months ago. Does this period affect results?

Can preservation of tissues in formalin 10 % for 3 months affect immunohistochemistry results?

03 April 2013 4,034 7 View

cDNA stability while shipping in RT for three days from one country to another?

I want to ship viral cDNA to the US for qRT-PCR that will take three days at room temperature. It will later be used for qRT-PCR. To what degree might it be degraded, or could it remain stable?

03 April 2013 8,672 19 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Muhammad Imran

Hi Ibrahim......Plz post your question in the cloning and related sections here on RG.......

Ibrahim Thabet

Thanks Imran, I did

Hamed Haghshenas

The following tutorial introduce you to many of the capabilities of InforMax’s Vector NTI.

http://www.basic.northwestern.edu/vectornti/documentation/vectornti/overview.htm

Thanks, Hamed but I need more detailed one, especially if it is a video, also your link is a good one