Ibrahim Thabet

9 Questions 71 Answers 0 Followers

Questions related from Ibrahim Thabet

Open Reading Frame (ORF)/Vector NTI?

How do I decide which is the right ORF (Open Reading Frame) for the translation using vector NTI software?

04 April 2017 6,041 5 View

I am preparing my manuscript for publication. Any recommendations for free publishing, as funding is very limited?

My paper is on the influenza virus. Do you know of any free peer reviewed journals in the area of Virology where I could publish?

12 December 2013 7,352 4 View

What is the importance of standard curve for RT-qPCR, I did standard curve for M gene H5N9 (known PFU), compared it to H5N1 M gene, is this correct?

11 November 2013 3,489 9 View

How can you solve a problem of non specific bands at RT-PCR, you expect at 700 bp and you get clear band at 500 - can anybody help?

09 September 2013 609 21 View

Does anybody have experience with using Roch PCR kits?

I do not know why my samples give good results with TAKARA protocol and not for Roch protocol. Roch High fidelity enzyme.

08 August 2013 8,836 0 View

Does anybody know what the mechanism of action of RLP lysis buffer of Qiagen kits is?

By which mechanism do they extract RNA, and why is RNA in this lysate very stable ?

08 August 2013 547 15 View

Does anybody here have a good link for tutorials in using vector NTI software for sequence analysis?

Vector NTI invitrogen.

08 August 2013 6,204 4 View

I want to prepare formalin fixed blocks for immunohistochemistry, samples preserved in formalin 10 % from 3 months ago. Does this period affect results?

Can preservation of tissues in formalin 10 % for 3 months affect immunohistochemistry results?

04 April 2013 4,132 7 View

cDNA stability while shipping in RT for three days from one country to another?

I want to ship viral cDNA to the US for qRT-PCR that will take three days at room temperature. It will later be used for qRT-PCR. To what degree might it be degraded, or could it remain stable?

04 April 2013 8,751 19 View