I do not know why my samples give good results with TAKARA protocol and not for Roch protocol. Roch High fidelity enzyme.
How do I decide which is the right ORF (Open Reading Frame) for the translation using vector NTI software?
03 April 2017 5,910 5 View
My paper is on the influenza virus. Do you know of any free peer reviewed journals in the area of Virology where I could publish?
11 December 2013 7,255 4 View
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10 November 2013 3,383 9 View
08 September 2013 512 21 View
Vector NTI invitrogen.
07 August 2013 6,127 4 View
By which mechanism do they extract RNA, and why is RNA in this lysate very stable ?
07 August 2013 450 15 View
Can preservation of tissues in formalin 10 % for 3 months affect immunohistochemistry results?
03 April 2013 4,034 7 View
I want to ship viral cDNA to the US for qRT-PCR that will take three days at room temperature. It will later be used for qRT-PCR. To what degree might it be degraded, or could it remain stable?
03 April 2013 8,672 19 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
25 February 2021 5,712 3 View