Open Reading Frame (ORF)/Vector NTI?

How do I decide which is the right ORF (Open Reading Frame) for the translation using vector NTI software?

03 April 2017 5,910 5 View

What is the importance of standard curve for RT-qPCR, I did standard curve for M gene H5N9 (known PFU), compared it to H5N1 M gene, is this correct?

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10 November 2013 3,383 9 View

How can you solve a problem of non specific bands at RT-PCR, you expect at 700 bp and you get clear band at 500 - can anybody help?

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08 September 2013 512 21 View

Does anybody here have a good link for tutorials in using vector NTI software for sequence analysis?

Vector NTI invitrogen.

07 August 2013 6,127 4 View

Does anybody have experience with using Roch PCR kits?

I do not know why my samples give good results with TAKARA protocol and not for Roch protocol. Roch High fidelity enzyme.

07 August 2013 8,740 0 View

Does anybody know what the mechanism of action of RLP lysis buffer of Qiagen kits is?

By which mechanism do they extract RNA, and why is RNA in this lysate very stable ?

07 August 2013 450 15 View

I want to prepare formalin fixed blocks for immunohistochemistry, samples preserved in formalin 10 % from 3 months ago. Does this period affect results?

Can preservation of tissues in formalin 10 % for 3 months affect immunohistochemistry results?

03 April 2013 4,034 7 View

cDNA stability while shipping in RT for three days from one country to another?

I want to ship viral cDNA to the US for qRT-PCR that will take three days at room temperature. It will later be used for qRT-PCR. To what degree might it be degraded, or could it remain stable?

03 April 2013 8,672 19 View

No title

02 March 2021 3,060 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Which period is considered as study period in research?

Is that starting from proposal development to result writeup or just from data collection to result writeup and reporting (publishing)?

28 February 2021 910 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View