Purification of AAV by ion exchange chromatography. How to adjust pH of virus lysate?

I would like to purify AAV particles using ion exchange chromatography. I am testing different pH levels for elution. Is it ok (safe for the virus particles) to use HCL or NaoH to...

08 September 2018 9,089 1 View

AAV purification protocol using chromatorgraphy columns for serotype 8?

Does anyone have a protocol (that has worked) or can suggest a protocol of AAV8 purification using an ion exchange column or any type of chromatography. I am looking for a procedure that does not...

31 December 2017 6,120 1 View

Density gradient- and serotype-independent method of AAV purification?

Dear all, Has anyone tried purifying recombinant AAV virus without density gradient ultracentrifugation in a serotype indepedent manner. Also, Is there a way to achieve high titer without...

11 December 2017 505 4 View

Virus particle purification?

I am transfecting T293 cells with pAAV (adeno-associated virus vector). How can I purify the recombinant virus after transfection?

01 February 2016 368 4 View

What is the best/cost-effective/fastest method for determining the titer of AAV?

So, I have transfected 293HEK cells with AAV-GFP vector and after purification, I ended up with 50ul of purified virus (or so I hope). What would be the best method to determine my titer with the...

01 February 2016 5,015 5 View

Can someone help with the tubular structures formed by bunyaviruses during their maturation?

Are these structures part of the Golgi membrane itself? or are they independent viral structures?

08 September 2015 1,308 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View