Purification of AAV by ion exchange chromatography. How to adjust pH of virus lysate?

09 September 2018 1 9K Report

I would like to purify AAV particles using ion exchange chromatography. I am testing different pH levels for elution. Is it ok (safe for the virus particles) to use HCL or NaoH to increase/decrease the pH of my virus solution before loading onto the column? Or do I need to use other buffers for that?

Thanks

Yamak Mehta

http://www.bio-rad.com/webroot/web/pdf/psd/literature/Bulletin_6807.pdf

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